Potensi ekstrak buah ramania (Bouea macrophylla Griffith) sebagai antibakteri terhadap beberapa bakteri patogen [The potential of ramania fruit extract (Bouea macrophylla Griffith) as an antibacterial against several pathogenic bacteria]

Nadra Khairiah, S.Si., Rais Salim

Abstract


Ramania fruit (Bouea macrophylla Griffith) is a tropical plants, as a new natural source of polyphenols. The content of these polyphenols is very abundant, especially in the seeds. The polyphenol content is widely used in the pharmaceutical world, as antibacterial ingredient. The development of natural antibacterial compounds from plants is needed to be used as a therapeutic treatment for bacterial infectious diseases. The purpose of this study is to determine the potential of ramania seed extract, seed coat and pulp against several types of pathogenic bacteria (Escherichia coli, Enterococcus aerogenes, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella thypimurium, and Propionibacterium acnes) that generally cause disease in humans. Ramania fruit is divided into three parts, such asseeds, seed coatand pulp. Antibacterial testing was done by agar diffusion method. The concentration of the extract for antibacterial tests were 1000 ppm, 500 ppm and 250 ppm.The MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) values were calculated using the microdilution method using 96-well micro-titre plates. The results showed that ramania seed extract had the highest antibacterial activity compared to seed coat extract and pulp. Inhibitory zone of Ramania seed extract ranged from 23-30 mm at a concentration of 1000 ppm and still had inhibition effect up to concentration of 250 ppm. MIC value of seed extract with methanol solvent was 78.13 μg/mL against three types of bacteria (P. aeruginosa, P. acnes, and S. thypimurium).


Keywords


antibakteri; bakteri patogen; Bouea macrophylla; ramania

Full Text:

PDF (Indonesian)

References


Adam, Z., Hassali, H. A., & Razali, R. (2016). Phytochemicals content, antioxidant and α-glucosidase inhibition activity of Bouea macrophylla Griff seed extract. Research and Development Seminar 2016, 1–8.

Dechsupa, N., Kantapan, J., Tungjai, M., & Intorasoot, S. (2019). Maprang “Bouea macrophylla Griffith” seeds: proximate composition, HPLC fingerprint, and antioxidation, anticancer and antimicrobial properties of ethanolic seed extracts. Heliyon, 5(7), 1–12. https://doi.org/10.1016/j.heliyon.2019.e02052.

Fitrya, F., Anwar, L., & Novitasari, E. (2010). Isolasi senyawa fenolat dari fraksi etil asetat kulit batang tumbuhan gandaria. Jurnal Penelitian Sains, 13(1), 10–14.

Ikram, M., Jan, G., Dad, S., Nasrullah,Suliman, & Fazl, H. (2013). Antimicrobial activities of the ethanol extracts of Mirabilis jalapa L. and Euphorbia dendroide. International Journal of Phytomedicine, 5, 475–478.

Jiamboonsri, P., Pithayanukul, P., Bavovada, R., & Chomnawang, M. T. (2011). The inhibitory potential of thai mango seed kernel extract against methicillin-resistant Staphylococcus aureus. Molecules, 16(8), 6255–6270. https://doi.org/10.3390/molecules16086255

Kabuki, T., Nakajima, H., Arai, M., Ueda, S., Kuwabara, Y., & Dosako, S. (2000). Characterization of novel antimicrobial compounds from mango (Mangifera indica L.) kernel seeds. Food Chemistry, 71(1), 61–66. https://doi.org/10.1016/S0308-8146(00)00126-6

Lolaen, L. A. C., Fatimawali, & Citraningtyas, G. (2013). Uji aktivitas antioksidan kandungan fitokimia jus buah gandaria (Bouea macrophylla Griffith). Jurnal Ilmiah Farmasi, 2(02), 1–8.

Mutua, J. K., Imathiu, S., & Owino, W. (2017). Evaluation of the proximate composition, antioxidant potential, and antimicrobial activity of mango seed kernel extracts. Food Science and Nutrition, 5(2), 349–357. https://doi.org/10.1002/fsn3.399

Orijajogun, J., Batari, L., Aguzue, O.,& Aguzue, O. (2014). Chemical composition and phytochemical properties of mango (Mangifera indica) seed kernel. International Journal of Advanced Chemistry, 2(2), 185. https://doi.org/10.14419/ijac.v2i2.3549

Raja, R. D. A., Jeeva, S., Prakash, J. W., Antonisamy, J. M., & Irudayaraj, V. (2011). Antibacterial activity of selected ethnomedicinal plants from South India. Asian Pacific Journal of Tropical Medicine, 4(5), 375–378. https://doi.org/10.1016/S1995-7645(11)60107-7

Rajan, N. S., & Bhat, R. (2017). Volatile constituents of unripe and ripe kundang fruits (Bouea macrophylla Griffith). International Journal of Food Properties, 20(8), 1751–1760. https://doi.org/10.1080/10942912.2016.1218892

Rajan, N. S., Bhat, R., & Karim, A. A. (2014). Preliminary studies on the evaluation of nutritional composition of unripe and ripe “Kundang” fruits (Bouea macrophyllaGriffith). International Food Research Journal, 21(3), 949–954.

Sheeba, E. (2010). Antibacterial activity of Solanum surattenseBurm. F. Kathmandu University Journal of Science, Engineering and Technology, 6(1), 1–4. https://doi.org/10.3126/kuset.v6i1.3278

Sukalingam, K. (2018). Preliminary phytochemical analysis and in vitro antioxidant properties of Malaysian „Kundang‟ (Bouea macrophylla Griffith). Journal of Food Biochemistry, 43(6), 261–266. https://doi.org/10.1111/jfbc.12913

Susanto, D., Sudrajat, & Ruga, R. (2012). Studi kandungan bahan aktif tumbuhan meranti merah (Shorea leprosula Miq) sebagai sumber senyawa antibakteri. Mulawarman Scientifie, 11(2), 181–190.

Tanasale, V. L. (2011). Kajian agronomi dan pemanfaatan buah gandaria (Bouea macrophylla Griff). Agrikan: Jurnal Ilmiah Agribisnis dan Perikanan, 4(2), 69–74. https://doi.org/10.29239/j.agrikan.4.2.69-74

Weinstein, M. P., Patel, J. B., Burnham, C-A., Campeau, S., Conville, P. S., Doern, C., ... Zimmer, B. L. (2018). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Wayne, PY: Clinical and Laboratory Standards Institute.




DOI: http://dx.doi.org/10.24111/jrihh.v12i2.6277

Refbacks

  • There are currently no refbacks.




JRIHH INDEXED BY :

       
       


Published by BARISTAND INDUSTRI BANJARBARU (E-ISSN: 2503-0779 dan P-ISSN : 2086-1400).

 Creative Commons License