PENGEMBANGAN BIOPLASTIK ANTIBAKTERI Morgonella morganii SEBAGAI KEMASAN MAKANAN

Eviomitta Rizki Amanda, Khoirun Nisyak, Yulianto Ade Prasetya

Abstract


Bioplastik antibakteri berbahan komposit biopolimer agarosa-kitosan telah berhasil dikembangkan dengan menggunakan gliserol sebagai plastisiser dan emulsi minyak serai dapur sebagai agen antibakteri. Komposit plastik­ agarosa-kitosan-emulsi minyak serai dapur dibuat dengan menggunakan metode casting. Beberapa konsentrasi emulsi minyak serai dapur seperti 1%v/v, 2%v/v, 3%v/v, 4%v/v, dan 5%v/v ditambahkan ke dalam larutan biopolimer dan dioptimasi dengan mengamati  daya hambatnya terhadap bakteri Morganella morganii dengan konsentrasi 106 cell/mL. Hasil optimasi menunjukkan bahwa konsentrasi optimum emulsi minyak serai dapur dalam larutan biopolimer untuk menghambat bakteri Morganella amorganii adalah 1%v/v dengan luas area daya hambat sebesar 16 cm2. Komposisi bioplastik yang optimum kemudian dikarakterisasi untuk mengetahui sifat fisik dan mekaniknya. Hasil pengukuran menunjukkan bahwa ketebalan komposit bioplastik dengan konsentrasi emulsi minyak serai 1% sebesar 0,0225 mm mengalami peningkatan sebesar 70% sampai 80% dari biopolimer agarosa dan kitosan secara terpisah. Uji sifat fisik dan mekanik dari bioplastik ditunjukkan oleh besarnya tensile strength (TS) dan elongation break (EB) yakni sebesar 51,28 MPa dan 4%, serta nilai water vapor permeability (WVP) bioplastik menggunakan emulsi minyak serai 1% sebesar 0,0097 g/cm2hari yakni lebih kecil dari WVP agarosa dan kitosan secara terpisah. Morfologi permukaan plastik dianalisis menggunakan Scanning Electron Microscopy (SEM) dan menunjukkan permukaan yang halus dan homogen. 


Keywords


Kemasan Antibakteri, Agarosa, Kitosan, Emulsi Minyak Serai Dapur, Morganella morganii

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References


Acevedo-Fani, Alejandra, Salvia-Trujillo, Laura, Rojas-Graü, María Alejandra, Martín-Belloso, and Olga. 2015. “Edible Films from Essential-Oil-Loaded Nanoemulsions: Physicochemical Characterization and Antimicrobial Properties.” Food Hydrocolloids 47.

Arswendiyumna, R. 2010. “Minyak Atsiri Daun Dan Batang Tanaman Dua Spesies Genus Cymbopogon, Famili Geraminae Sebagai Insektisida Alami Dan Antibaktei.” ITS, Surabaya.

Balakrishnan, Balachandar, Sadayan Paramasivam, and Abimanan Arulkumar. 2014. “Evaluation of the Lemongrass Plant (Cymbopogon Citratus) Extracted in Different Solvents for Antioxidant and Antibacterial Activity against Human Pathogens.” Asian Pacific Journal of Tropical Disease 4 (S1).

Gericke, M, and T Heinze. 2015. “Homogeneous Tosylation of Agarose as an Approachtoward Novel Functional Polysaccharide Materials.” Carbohyd. Polym 127:236–45.

Guerrero, P., A. Etxabide, I. Leceta, M. Pe˜nalba, and K. De la Caba. 2014. “Extraction of Agarfrom Gelidium Sesquipedale (Rodhopyta) and Surface Characterization of Agarbased Films.” Carbohyd. Polym 99:491–98.

Habibi, P, M Sabaghi, and Y Maghsoudlou. 2015. “Enhancing Structural Properties Andantioxidant Activity of Kefiran Films by Chitosan Addition.” Food Struct 5:66–71.

Han, Yingying, Miao Yu, and Lijuan Wang. 2017. “Physical and Antimicrobial Properties of Sodium Alginate / Carboxymethyl Cellulose Fi Lms Incorporated with Cinnamon Essential Oil.” Food Packaging and Shelf Life.

Hanaa, A. R. M., Y.I. Sallam, A.S. El-Leithy, and S.E. Aly. 2012. “Lemongrass (Cymbopogon Citratus) Essential Oil as Affected by Drying Methods.” Annals of Agricultural Science 72 (2):113–16.

Hu, Zhang, Pengzhi Hong, Liao Mingneng, Songzhi Kong, Na Huang, and Chunyan Ou. 2016. “Preparation and Characterization of Chitosan—Agarose Composite Films.” Materials 9 (816):1–9.

Jiang S, Peng Y, Ning B, Bai J, Liu Y, Zhang N, et al. 2015. Chemical Surface plasmon resonance sensor based on molecularly imprinted polymer film for detection of histamine. Sensors Actuators B Chem. 221:15–21.

Lagaron, J.M., and M.J. Ocio P. Fernandez-Saiz. 2007. “Using ATR-FTIR Spectroscopy Todesign Active Antimicrobial Food Packaging Structures Based on Highmolecular Weight Chitosan Polysaccharide.” J. Agr. Food Chem 55 (7):2554–62.

Li, G, and G Yuan. 2016. “Chitosan Films and Coatings Containing Essential Oils:the Antioxidant and Antimicrobial Activity and Application in Food Systems.” Food Res. Int 89:117–28.

Nasri, M, M Jridi, S Hajji, H.B Ayed, I Lassoued, A Mbarek, and M Kammoun. 2014. “Physical, Structural, Antioxidant and Antimicrobial Properties Ofgelatin-Chitosan Composite Edible Films.” Int. J. Biol. Macromol 67:373–79.

Ng, N, T, M Sanagi, M, W Ibrahim, W, N, and W Ibrahim, W, A. 2017. “Agarose-Chitosan-C18 Film Micro Solid Phase Extraction with High Performance Liquid Chriomatography for the Determination of Phenantrene and Pyrene in Chrysanthemum Tea.” Food Chemistry 222:28–34.

Nyoman Semadi Antara, Dwi Ayu Kirani Paramita, Anak Agung Duwipayana, Ida Bagus Wayan Gunam. 2016. “Inhibitory Activity Of Lemongrass Essential Oil Against Eschericia Coli, Staphylococcus Aureus, And Vibrio Cholera.” Anali. Chim. Acta 945:47–56.

Qiqi, Cao, Zhang Yi, Chen Wei, Meng Xianghong, and Liua Bingjie. 2018. “Hydrophobicity and Physicochemical Properties of Agarose Film as Affected by Chitosan Addition.” International Journal of Biological Macromolecules 106.

Ren, L., X. Yan, J. Zhou, J. Tong, and X. Su. 2017. “Influence of Chitosan Concentration Onmechanical and Barrier Properties of Corn Starch/chitosan Films,.” Int. J. Biol.Macromol.

Singh, Bhoj Raj, Vidya Singh, Raj Karan Singh, and N Ebibeni. 2011. “Antimicrobial Activity of Lemongrass ( Cymbopogon Citratus ) Oil against Microbes of Environmental , Clinical and Food Origin,”.

Verma, V, A Wadhiya, and D Kumar. 2016. “Crosslinking of Agarose Bioplastic Usingcitric Acid,.” Carbohyd. Polym 151:60–67.

Yaksan, R, and K.M Dang. 2015. “Development of Thermoplastic Starch Blown Film Byincorporating Plasticized Chitosan.” Carbohyd. Polym 115:575–81.

Yuen, S.N., S.M. Choi, D.L. Phillips, C.Y., and Ma. 2009. “Raman and FTIR Spectroscopicstudy of Carboxymethylated Non-Starch Polysaccharides.” Food Chemistry 114 (3):1091–98.




DOI: http://dx.doi.org/10.24817/jkk.v42i1.5745

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