AKTIVITAS ANTIBAKTERI DAN UJI EFIKASI IN VIVO MEMBRAN SERAT NANO POLIVINIL ALKOHOL/GELATIN DENGAN ANTIBIOTIKA TOPIKAL UNTUK TEKSTIL MEDIS PEMBALUT LUKA

Eva Novarini, Theresia Mutia, Rr. Srie Gustiani

Abstract


Luka memiliki beberapa derajat keparahan dan terkadang memerlukan penanganan khusus, misalnya untuk luka kronis akibat diabetes atau borok vena. Pembalut luka dari membran serat nano campuran polimer sintetik polivinil alkohol (PVA) dan polimer alam gelatin dapat dijadikan pembalut untuk luka kronis. Penelitian ini bertujuan untuk membuktikan adanya aktivitas antibakteri dan kaitannya dengan penyembuhan luka oleh membran serat nano PVA/gelatin yang ditambahkan antibiotika topikal Bacitracin dan Neomycin. Penilaian aktivitas antibakteri dilakukan dengan metode parallel streak menggunakan bakteri E. coli dan S. aureus. Untuk mengetahui efikasi membran serat nano terhadap penyembuhan luka terinfeksi, dilakukan uji in vivo menggunakan kelinci albino jantan sehat yang diinfeksi oleh bakteri E. coli dan S. aureus. Hasil pengujian menyatakan bahwa membran serat nano memiliki kekuatan antibakteri tinggi dilihat dari zona hambat/daerah bening > 20 mm yang menandakan mikroorganisme sangat sensitif terhadap spesimen uji. Membran serat nano juga terbukti dapat mempercepat penyembuhan luka terinfeksi. Kelinci albino yang diinfeksi oleh bakteri dan diobati membran serat nano menunjukkan kesembuhan total pada hari ke 5 sedangkan kelinci kontrol sembuh pada hari ke 14. Berdasarkan hasil yang ada, membran serat nano PVA/gelatin/antibiotika topikal ini berpotensi untuk dijadikan sebagai pembalut luka terinfeksi.

Keywords


Keywords: nanofibrous membrane, wound dressing, gelatin, topical antibiotics, in vivo studies

Full Text:

PDF

References


Ahlawat, J., Kumar, V. & Gopinath, P. Carica papaya loaded poly (vinyl alcohol)-gelatin nanofibrous scaffold for potential application in wound dressing. Mater. Sci. Eng. C 103, 109834 (2019).

Bakhsheshi-Rad, H. R. et al. Antibacterial activity and in vivo wound healing evaluation of polycaprolactone-gelatin methacryloyl-cephalexin electrospun nanofibrous. Mater. Lett. 256, 126618 (2019).

Gu, S. Y., Wang, Z. M., Ren, J. & Zhang, C. Y. Electrospinning of gelatin and gelatin/poly(l-lactide) blend and its characteristics for wound dressing. Mater. Sci. Eng. C 29, 1822–1828 (2009).

Gizaw, M. et al. Electrospun fibers as a dressing material for drug and biological agent delivery in wound healing applications. Bioengineering 5, 1–28 (2018).

Sidgwick, G. P., McGeorge, D. & Bayat, A. A comprehensive evidence-based review on the role of topicals and dressings in the management of skin scarring. Arch. Dermatol. Res. 307, 461–477 (2015).

Amler, E. et al. Functionalized nanofibers as drug-delivery systems for osteochondral regeneration. Nanomedicine 9, 1083–1094 (2014).

İnal2019-Production and characterization of bactericidal wound dressing material based on gelatin nanofiber.pdf.

Chong, E. J. et al. Evaluation of electrospun PCL/gelatin nanofibrous scaffold for wound healing and layered dermal reconstitution. Acta Biomater. 3, 321–330 (2007).

Liu, Y., Zhou, S., Gao, Y. & Zhai, Y. Electrospun nanofibers as a wound dressing for treating diabetic foot ulcer. Asian J. Pharm. Sci. 14, 130–143 (2019).

Kamoun, E. A., Kenawy, E. R. S. & Chen, X. A review on polymeric hydrogel membranes for wound dressing applications: PVA-based hydrogel dressings. J. Adv. Res. 8, 217–233 (2017).

Mir, M. et al. Synthetic polymeric biomaterials for wound healing: a review. Prog. Biomater. 7, 1–21 (2018).

Gaspar-Pintiliescu, A., Stanciuc, A. M. & Craciunescu, O. Natural composite dressings based on collagen, gelatin and plant bioactive compounds for wound healing: A review. Int. J. Biol. Macromol. 138, 854–865 (2019).

Mutia, T., Novarini, E. & Gustiani, Srie, R. Preparasi dan Karakterisasi Membran Serat Nano Polivinil Alkohol/Gelatin Dengan Antibiotika Topikal Menggunakan Metode Electrospinning. Arena Tekst. (2020).

Huang, C. Y., Hu, K. H. & Wei, Z. H. Comparison of cell behavior on pva/pva-gelatin electrospun nanofibers with random and aligned configuration. Sci. Rep. 6, (2016).

Dias, J. R. et al. In situ crosslinked electrospun gelatin nanofibers for skin regeneration. Eur. Polym. J. 95, 161–173 (2017).

Legnoverde, M. S. & Basaldella, E. I. Influence of particle size on the adsorption and release of cephalexin encapsulated in mesoporous silica SBA-15. Mater. Lett. 181, 331–334 (2016).

Talebian, A. & Mansourian, A. Release of Vancomycin from electrospun gelatin/chitosan nanofibers. Mater. Today Proc. 4, 7065–7069 (2017).

Huang, C. H. et al. Evaluation of proanthocyanidin-crosslinked electrospun gelatin nanofibers for drug delivering system. Mater. Sci. Eng. C 32, 2476–2483 (2012).

Morsy, R., Hosny, M., Reicha, F. & Elnimr, T. Developing a potential antibacterial long-term degradable electrospun gelatin-based composites mats for wound dressing applications. React. Funct. Polym. 114, 8–12 (2017).

Nitanan, T., Akkaramongkolporn, P., Rojanarata, T., Ngawhirunpat, T. & Opanasopit, P. Neomycin-loaded poly(styrene sulfonic acid-co-maleic acid) (PSSA-MA)/polyvinyl alcohol (PVA) ion exchange nanofibers for wound dressing materials. Int. J. Pharm. 448, 71–78 (2013).

Boateng, J. & Catanzano, O. Advanced Therapeutic Dressings for Effective Wound Healing - A Review. J. Pharm. Sci. 104, 3653–3680 (2015).

Bommarito, L., Mietta, S. & Cadario, G. Anaphylaxis after application of topical bacitracin-neomycin powder. Ann. Allergy, Asthma Immunol. 115, 74–75 (2015).

O’Donnell, J. A., Gelone, S. P. & Safdar, A. Topical Antibacterials. Mandell, Douglas, and Bennett’s Principles and Practice of Infectious Diseases vol. 1 (Elsevier Inc., 2014).

Chemistry, B. Neomycin. 1–5 (2007).

BPOM. Peraturan Kepala Badan Pengawas Obat Dan Makanan Republik Indonesia Nomor 7 Tahun 2014. Tentang Pedoman Uji Toksisitas Nonklinik Secara Vivo 66–68 (2014).

AATCC. AATCC Test Method 147-2004 Antibacterial Activity Assessment of Textile Materials: Parallel Streak Method. (2004).

Jawelz, M. Mikrobiologi Kedokteran. (EGC, 1995).

Anonimous. Informasi Spesialite Obat Indonesia. (2008).

Hayes, A. Principles and Method of Toxicology. (Raven Press Ltd, 1989).

Organization for Economic Cooperation and Development, O. OECD 404 Guidelines for Testing of Chemicals – Acute Dermal Irritation/Corrotion. (2002).

Mikrobiologi Kedokteran. (McGraw Hill, 2004).

Hong, W., Gao, X. & Qiu, P. Corrigendum: Synthesis, construction, and evaluation of self-assembled nano-bacitracin a as an efficient antibacterial agent in vitro and in vivo (Int J Nanomedicine. 2017, 12, 4691-4708.). Int. J. Nanomedicine 15, 4753 (2020).

PDR. Prescriber Digital Reference. Neomycin Sulfate - Drug Summary.

Blanchard, C. et al. Neomycin sulfate improves the antimicrobial activity of mupirocin-based antibacterial ointments. Antimicrob. Agents Chemother. 60, 862–872 (2016).

Rajendran, S. Advanced textiles for wound care. Advanced Textiles for Wound Care (2009). doi:10.1533/9781845696306.

Paul, W. & Sharma, C. P. Advances in Wound Healing Materials : Science and Skin Engineering. A Smithers Group Company Shawbury, (2015).




DOI: http://dx.doi.org/10.31266/at.v36i1.6708

Refbacks

  • There are currently no refbacks.


Arena Tekstil indexed by:

Science and Technology Index  Dimensions

 Garba Rujukan Digital (GARUDA)


Copyright Arena Tekstil (E-ISSN: 2548-7264, P-ISSN: 0518-4010)

Published by: BALAI BESAR TEKSTIL, Jl. Jenderal Ahmad Yani No. 390, Bandung.

Creative Commons License