Potensi Ekstrak Air Angkak dalam Mencegah Peroksidasi Lipid dan Efeknya terhadap Organ Hati Tikus Sprague Dawley

. Hasim, Novena Nathasia, Agus Setiyono, Didah Nur Faridah

Abstract


Angkak (red yeast rice), bahan pangan fermentasi beras oleh kapang Monascus purpureus dan memiliki beberapa senyawa metabolit bersifat antioksidan.  Penelitian ini bertujuan menganalisis potensi ekstrak air angkak dalam mencegah peroksidasi lipid di dalam serum dan organ hati tikus.  Sprague Dawley yang diinduksi propiltiourasil dan diet tinggi lemak. Tikus sebanyak 25 ekor dibagi menjadi lima kelompok [normal, kontrol positif, kontrol negatif, induksi air angkak 50 mg/kg (kelompok A1), dan 100 mg/kg (kelompok A2)]. Parameter uji yang diamati adalah konsentrasi malonaldehid (MDA) serum darah dan hati, aktivitas enzim aminotransferase serum, dan gambaran mikroskopik hati. Tikus dengan induksi air angkak 100 mg/kg menunjukkan penghambatan peningkatan konsentrasi MDA pada serum dan hati serta aktivitas AST signifikan terhadap kelompok kontrol negatif. Histopatologi hati kelompok yang induksi ekstrak air angkak menunjukkan adanya perbaikan jaringan yang secara kualitatif ditandai dengan sel hepatosit utuh yang diwarnai oleh pewarna Hematoksilin-eosin, susunan sel rapat dan berjajar secara radial. Induksi 100 mg/kg masih menunjukkan perbesaran vakuola atau butiran lipid yang berwarna putih yang menjadi penanda degenerasi lemak.

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DOI: http://dx.doi.org/10.32765/warta%20ihp.v37i1.5438

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